Journal: Advanced Science

Article Title: Black Phosphorus Accelerates Bone Regeneration Based on Immunoregulation

doi: 10.1002/advs.202304824

Figure Lengend Snippet: BP regulates immune signaling in bone regeneration. A) A KEGG enrichment analysis of differentially expressed genes between PCP and PCC groups. B) A summary of the expression and role of immune molecules associated with bone regeneration. C) A PPI analysis of the interactions of immune molecules associated with bone regeneration. D) IF staining images of local pro‐inflammatory factors, TNF‐ α and IL‐6, and anti‐inflammatory factors, IL‐10 and IGF1, at 1 and 2 weeks after PCC and PCP scaffold implantation. S, scaffold. E) Analysis of mean grays value of IF staining, n = 5.

Article Snippet: The following primary antibodies were used: OCN (1:200, MAB1419, R&D), COL‐1 (1:200, ab270993, Abcam), TNF‐ α (1:200, ab205587, Abcam), IL‐6 (1:200, AF506, R&D), IL‐10 (1:200, AF519, R&D), IGF‐1 (1:200, NBP2‐44968, NOVUS), IL‐33 (1:200, ab187060, Abcam), CD169 (1:200, ab312840, Abcam), ALP (1:200, NB110‐3638, NOVUS), and BMP2 (1:200, ab284387, Abcam).

Techniques: Expressing, Staining

Journal: Frontiers in Oncology

Article Title: Growth hormone receptor antagonism downregulates ATP-binding cassette transporters contributing to improved drug efficacy against melanoma and hepatocarcinoma in vivo

doi: 10.3389/fonc.2022.936145

Figure Lengend Snippet: GHRA suppresses syngeneic mouse melanoma growth in vivo . (A) Mouse Fluc-B16-F10 cells grafted intradermally on the right flank of syngeneic C57BL6/J wild-type (WT) and GHA male mice (transgenic for bGH G119K GHR antagonist) (n=6). Tumor growth over 4-weeks was followed by luminescent imaging following luciferin injections. Luciferin signal was quantified and plotted on the right. The changes in tumor volume (Fluc-B16-F10 in WT and GHA mice) from digital caliper measurement (B) and tumor mass (C) corroborate the suppressed tumor growth in GHA mice which has markedly lower serum IGF1 levels (D) due to presence of a circulating GHRA. Western-blot analysis of the GH downstream signaling mediators – phosphorylated STAT5, AKT and SRC kinase in the tumors of GHA and WT mice (E) . B16-F10 cells in culture when treated for 72-hours with serum collected from WT and GHA mice showed suppressed growth rate in the GHA mouse serum (F) . (*p < 0.05, mouse studies – repeated measure using SPSS; cell viability - Students t test, n = 3).

Article Snippet: The sera were then collected and measured by ELISA for IGF1 (#22-IG1MS-E01, ALPCO, Salem, NH, USA) according to the manufacture’s guidelines.

Techniques: In Vivo, Transgenic Assay, Imaging, Western Blot

Journal: Frontiers in Oncology

Article Title: Growth hormone receptor antagonism downregulates ATP-binding cassette transporters contributing to improved drug efficacy against melanoma and hepatocarcinoma in vivo

doi: 10.3389/fonc.2022.936145

Figure Lengend Snippet: Effect of GHRA on response of syngeneic mouse hepatocellular carcinoma tumors to sorafenib treatment in vivo. (A) Mouse Hepa1-6 hepatocellular carcinoma (HCC) cells respond to bovine GH stimulation in culture showing increased cell viability and STAT5 activation after 72 hours (A) as well as increase in the EC50 dose of sorafenib (B) . Additionally, GH treatment increased ABC transporter levels in the cultured Hepa1-6 cells (C) . Mouse Hepa1-6 cells grafted subcutaneously on the right flank of syngeneic C57BL6/J wild-type (WT) and GHA mice (transgenic for bGH G119K GHR antagonist) (n=6). The changes in tumor volume (Hepa1-6 in WT and GHA mice) from digital caliper measurement (D) and post-dissection tumor mass (E) show suppressed tumor growth and improved tumoral response to sorafenib in the GHA mice. (F) Spearman correlation analysis for transcript levels of GHR and ABC transporter and IGF1R and ABC transporters in the tumor of 371 HCC patients in the TCGA cohort. (G) Serum IGF1 levels of Hepa1-6 tumor-bearing GHA mice are significantly lower than that of tumor-bearing WT mice. (H–J) Correlation of overall survival probability of 371 HCC patients in the TCGA cohort with RNA expression of IGF1R (H) , or ABCB1 (I) , or ABCC1 (J) . (*p < 0.05, ***p < 0.001, mouse studies – repeated measure using SPSS; other assays - Students t test, n = 3).

Article Snippet: The sera were then collected and measured by ELISA for IGF1 (#22-IG1MS-E01, ALPCO, Salem, NH, USA) according to the manufacture’s guidelines.

Techniques: In Vivo, Activation Assay, Cell Culture, Transgenic Assay, Dissection, RNA Expression

Journal: Scientific Reports

Article Title: Insulin-like growth factor 2 and autophagy gene expression alteration arise as potential biomarkers in Parkinson’s disease

doi: 10.1038/s41598-022-05941-1

Figure Lengend Snippet: Plasma IGF1 and IGF2 levels in PD patients do not correlate with motor scores of PD patients. ( A ) Pearson correlation analysis between IGF2 levels in PD patients with H&Y (top panel, r = 0.1371), UPDRSIII (middle panel, r = − 0.07482) scores and evolution disease time (bottom panel, r = − 0.1951). ( B ) Correlation analysis between IGF1 levels in PD patients with H&Y (top panel, r = 0.1498), UPDRSIII (middle panel r = − 0.07823) scores and evolution disease time (bottom panel, r = − 0.09742).

Article Snippet: The plasma levels of IGF1 and IGF2 were measured by ELISA (ALPCO, 22-IGFHU-E01, and 22-IG2HU-E01) according to the manufacturer’s instructions.

Techniques: Clinical Proteomics